Forms for Flow Cytometry

Here are some Excel spreadsheets that may be useful to you in your daily lab work:

Form for designing multicolor Ab panels:  Download Ab panel config sheet
(delete rows for colors you don't use or that don't apply to your cytometer)

Form for hemacytometer cell counting & cell dilutions:  Download Cell Calculation Form
(enter information in the clear cells; yellow cells are auto-calculated for you)

Posted on 19 November 2008 in Forms and Tools | | Comments (0)

MIFlowCyt Templates

MIFlowCyt (Minimum Information about a Flow Cytometry Experiment) is a new ISAC-approved standard for what information should be included when publishing flow cytometry data (see Cytometry A, 2008, 73(10):926-30 and online supporting material).

The Flow Core at Baylor Institute of Immunology Research has several multicolor instruments for which I've created MIFlowCyt templates:


You can use these templates, which contain all default instrument information, to fill in the remaining information pertinent to your experiment.  Note that the templates contain information specific to the BIIR instruments; your cytometer may be different, but you can still use these as a starting point.

Posted on 19 November 2008 in Forms and Tools | | Comments (0)

Tools for Immune Function Assays

The following are some useful tools for immune function assays:

To calculate sample size required for statistical significance in intracellular cytokine assays:  Download sample_size_calc.xls

To determine a confidence interval around an intracellular cytokine result:  Download conf_interval_calc.xls

To create a list of 15 aa peptides, overlapping by 11 aa each, for a sequence of your choice:  Download Peptidechopper2.xls

Posted on 24 April 2006 in Forms and Tools | | Comments (0)