Here are some new protocols designed to cover basic areas of flow cytometry. These are intended as general guides, not specific SOPs, which you can generate according to your own needs. Cryopreservation of PBMC: Download Protocol-Cryopreservation.pdf Cell-surface staining for flow cytometry: Download protocolsurface_staining.pdf Activation for functional assays: Download Protocol-Activation.pdf Intracellular staining: Download protocolic_staining.pdf Experiment setup (for BD digital instruments): Download protocolexp_setup.pdf NEW Experiment setup (for BD analog instruments): Download protocolexp_setupanalog.pdf